ABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb,ABclonal,A24240

Reactivity

Species independent

Application

FC

Conjugate

ABflo® 594. Ex:588nm. Em:604nm.

Platform ID

BAB681629656

ABclonal

Headquarters

500W Cummings Park, Ste. 6500 Woburn, MA 01801

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Product Specifications
Scientific Background

Specifications

NameABflo® 594 Rabbit anti-FITC/5-FAM/6-FAM mAb
Cat. No.A24240
RRID#N/A
IsotypeIgG
ReactivitySpecies independent
ConjugationABflo® 594. Ex:588nm. Em:604nm.
ApplicationFC
Working DilutionsFC:5 μl per 10^6 cells in 100 μl volume
Clone NumberARC51071
ImmunogenChemical compounds corresponding to FITC/5-FAM/6-FAM.
PurityAffinity purification
Appearance/FormLiquid
StorageStore at 2-8℃. Avoid freeze.; Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Regulatory StatusResearch Use Only

Scientific Background

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

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