APC/Cyanine7 anti-mouse CD117 c-kit Antibody anti-CD117 - ACK2,BioLegend,135135

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB016070427

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameAPC/Cyanine7 anti-mouse CD117 c-kit Antibody anti-CD117 - ACK2
Cat. No.135135
HostRat
RRIDAB_2632808 (BioLegend Cat. No. 135135)AB_2632809 (BioLegend Cat. No. 135136)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberACK2
Concentration0.2 mg/ml
TargetCD117
ImmunogenMurine IL-3 dependent mast cells
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

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