APC/Cyanine7 anti-mouse Ly-6A/E Sca-1 Antibody anti-Ly-6A/E - D7,BioLegend,108126

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1,in vitrolymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13and Bouin-fixed, paraffin-embedded samples9.The two Sca-1 recognizing clones D7 andE13-161.7have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB217507484

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Product Specifications
Scientific Background

Specifications

NameAPC/Cyanine7 anti-mouse Ly-6A/E Sca-1 Antibody anti-Ly-6A/E - D7
Cat. No.108126
HostRat
RRIDAB_10639725 (BioLegend Cat. No. 108125)AB_10645327 (BioLegend Cat. No. 108126)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberD7
Concentration0.2 mg/ml
TargetLy-6A/E
ImmunogenIL-2-dependent mouse T-cell line (CTL-L)
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

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