APC/Fire 750 anti-human CD11b (activated) Antibody anti-CD11b - CBRM1/5,BioLegend,301420

The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9.Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).

Host

Mouse

Reactivity

Human

Application

FC -Quality tested

Platform ID

BAB027564907

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Product Specifications
Scientific Background

Specifications

NameAPC/Fire 750 anti-human CD11b (activated) Antibody anti-CD11b - CBRM1/5
Cat. No.301420
HostMouse
RRIDAB_2715898 (BioLegend Cat. No. 301419)AB_2715899 (BioLegend Cat. No. 301420)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberCBRM1/5
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD11b
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αMintegrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2(CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.

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