APC/Fire™ 750 anti-human CD40 Antibody, CD40, HB14,BioLegend,313021

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis.1Alone, or in combination with TLR ligands, clone HIB14 stimulates B cells to produce IL-10 and differentiates it into regulatory B10 (IL-10 producing B cells).7The Ultra-LEAF™purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 313019 & 313020).

Host

Mouse

Reactivity

Human, Cynomolgus, Rhesus

Application

FC -Quality tested

Platform ID

BAB353964482

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameAPC/Fire™ 750 anti-human CD40 Antibody, CD40, HB14
Cat. No.313021
HostMouse
RRIDAB_2860831 (BioLegend Cat. No. 313021)AB_2860832 (BioLegend Cat. No. 313022)
IsotypeMouse IgG1, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberHB14
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD40
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

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