APC/Fire™ 750 anti-mouse CD25 Antibody CD25, 3C7,BioLegend,101921
Additional reported applications (for the relevant formats) include:in vitroblocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.101925 and 101926).
Host
Rat
Reactivity
Mouse
Application
FC -Quality tested
Platform ID
BAB245005543
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameAPC/Fire™ 750 anti-mouse CD25 Antibody CD25, 3C7
Cat. No.101921
HostRat
RRIDAB_2783040 (BioLegend Cat. No. 101921)AB_2783041 (BioLegend Cat. No. 101922)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number3C7
Concentration0.2 mg/ml
TargetCD25
ImmunogenIL-2-dependent BALB/c mouse helper T-cell clone HT-2
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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