APC/Fire™ 750 anti-mouse CD49b (pan-NK cells) Antibody, CD49b, DX5,BioLegend,108925

The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagenin vitro.Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2and immunohistochemical staining5of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB595593502

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameAPC/Fire™ 750 anti-mouse CD49b (pan-NK cells) Antibody, CD49b, DX5
Cat. No.108925
HostRat
RRIDAB_2876423 (BioLegend Cat. No. 108925)AB_2876423 (BioLegend Cat. No. 108926)
IsotypeRat IgM, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberDX5
Concentration0.2 mg/mL
TargetCD49b
ImmunogenIL-2-propagated NK1.1+cells from C57BL/6 mice
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2integrin, VLA-2 α chain, and integrin α2chain. CD49b non-covalently associates with CD29 (β1integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

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