APC anti-human CD11b Antibody anti-CD11b - ICRF44,BioLegend,301350

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Host

Mouse

Reactivity

Human, Cynomolgus, Rhesus

Application

FC - Quality tested

Platform ID

BAB800528879

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameAPC anti-human CD11b Antibody anti-CD11b - ICRF44
Cat. No.301350
HostMouse
RRIDAB_314161 (BioLegend Cat. No. 301309)AB_314162 (BioLegend Cat. No. 301310)AB_2564134 (BioLegend Cat. No. 301350)
IsotypeMouse IgG1, κ
ReactivityHuman, Cynomolgus, Rhesus
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberICRF44
Concentrationµg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD11b
PurityThe antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Formulationµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αMintegrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2(CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.