APC anti-human CD45RO Antibody, CD45RO, S19021B,BioLegend,376810

The S19021B antibody can be used in combination with antibodies against CD45RA to discern memory and naïve T cells. Staining profile by S19021B on human peripheral blood lymphocyte is comparable to that by clone UCHL1.Staining signal by clone S19021B on target cells can be completely blocked by pre-incubation with clone UCHL1; staining of UCHL1 on target cells can be partially blocked by pre-incubation with clone S19021B.Staining signal by clone S19021B can be significantly increased on the cells after neuraminidase treatment, suggesting that S19021B binding epitope is likely affected by glycosylation.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB822207204

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAPC anti-human CD45RO Antibody, CD45RO, S19021B
Cat. No.376810
HostMouse
RRIDAB_3083369 (BioLegend Cat. No. 376809)AB_3083369 (BioLegend Cat. No. 376810)
IsotypeMouse IgG1, λ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberS19021B
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD45RO
ImmunogenSynthetic human CD45 peptide spanning the junction region between exons 3 and 7.
PurityThe antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

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