APC anti-human IL-10 Antibody anti-IL-10 - JES3-19F1,BioLegend,506806

ELISA or ELISPOT Capture1-4:The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.Flow Cytometry:The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.Neutralization1,2:The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).Additional reported applications (for the relevant formats) include:Western blotting, immunohistochemical staining5,6of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.Note:For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended.