APC anti-human IL-2 Antibody anti-IL-2 - MQ1-17H12,BioLegend,500309
ELISA or ELISPOT Capture2,3:The purified MQ1-17H12 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Biotin anti-human IL-2 antibody (Cat. No. 517605) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 4.0 - 8.0 µg/mL is recommended.Additional reported applications (for the relevant formats) include:immunoprecipitation2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,4-6,8, neutralization13, and immunocytochemistry.Note:For testing human IL-2 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 431807) is specially developed and recommended.Clone MQ1-17H12 cross-reacts to Cat15
Host
Rat
Reactivity
Human
Application
ICFC - Quality tested
Platform ID
BAB819619711
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameAPC anti-human IL-2 Antibody anti-IL-2 - MQ1-17H12
Cat. No.500309
HostRat
RRIDAB_315096 (BioLegend Cat. No. 500309)AB_315098 (BioLegend Cat. No. 500311)AB_315097 (BioLegend Cat. No. 500310)
IsotypeRat IgG2a, κ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberMQ1-17H12
Concentrationµg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-2
ImmunogenE. coli- expressed recombinant human IL-2
PurityThe antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Formulationtest sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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