APC anti-mouse CD126 IL-6Ralpha chain Antibody anti-CD126 - D7715A7,BioLegend,115812

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5,in vivoreceptor blocking1-2,4-6,8, andin vitroneutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined tonotreact with rat.

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB655276105

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Product Specifications
Scientific Background

Specifications

NameAPC anti-mouse CD126 IL-6Ralpha chain Antibody anti-CD126 - D7715A7
Cat. No.115812
HostRat
RRIDAB_2127937 (BioLegend Cat. No. 115811)AB_2296238 (BioLegend Cat. No. 115812)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberD7715A7
Concentration0.2 mg/ml
TargetCD126
ImmunogenOKT4 hybridoma cells
PurityThe antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

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