APC anti-mouse IL-12/IL-23 p40 monomer, dimer, heterodimer Antibody anti-IL-12/IL-23 p40 - C15.6,BioLegend,505206

ELISA or ELISPOT Capture1,2,4,6,8,10:The purified C15.6 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated C17.8 (Cat. No. 505302) antibody as the detecting antibody. This assay detects p40 as monomer, homodimer, or heterodimer complexed with p35. The LEAF™ purified antibody is suggested for ELISPOT capture.Additional reported applications (for the relevant formats) include:immunohistochemical staining9of paraformaldehyde-fixed saponin-treated frozen tissue sections, immunoprecipitation3, and Western blotting3.Note:For testing mouse IL-12 p40 (monomer, dimer, heteromer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended.

Host

Rat

Reactivity

Mouse

Application

ICFC - Quality tested

Platform ID

BAB963084608

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Product Specifications
Scientific Background

Specifications

NameAPC anti-mouse IL-12/IL-23 p40 monomer, dimer, heterodimer Antibody anti-IL-12/IL-23 p40 - C15.6
Cat. No.505206
HostRat
RRIDAB_315369 (BioLegend Cat. No. 505205)AB_315370 (BioLegend Cat. No. 505206)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberC15.6
Concentration0.2 mg/ml
TargetIL-12/IL-23 p40
ImmunogenCHO-expressed, recombinant mouse IL-12 p70
PurityThe antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The C15.6 antibody reacts with mouse IL-12 p40 subunit of the IL-12 p70 and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer, or heterodimer. The C15.6 antibody can not neutralize the bioactivity of natural or recombinant IL-12.

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