APC anti-mouse IRF5 Antibody, IRF5, W16007B,BioLegend,158605
This clone was tested for ICC using RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with IRF5 staining, the signal was weak and did not meet our sensitivity standards.This clone does not recognize human IRF5. For detection of human IRF5, please use BioLegend clone 11F4A09 (Cat. No. 652602).During product development testing of this clone for western blot, a ~37 kD protein of unknown origin was faintly detected. This protein may represent a minor IRF5 degradation product.
Host
Rat
Reactivity
Mouse
Application
ICFC - Quality tested
Platform ID
BAB203391734

BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameAPC anti-mouse IRF5 Antibody, IRF5, W16007B
Cat. No.158605
HostRat
RRIDAB_2924490 (BioLegend Cat. No. 158605)AB_2924490 (BioLegend Cat. No. 158606)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberW16007B
Concentration0.2 mg/mL
TargetIRF5
ImmunogenMouse IRF5 recombinant protein (149-400 a.a.)
PurityThe antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
The interferon regulatory factor (IRF) family of transcription factors comprises nine members with conserved domains. IRF5 mediates the induction of proinflammatory cytokine, such as IL-6 and TNFa. IRF5 is highly expressed in monocytes, macrophages, B cells, and dendritic cells. Expression of IRF5 is associated with many autoimmune diseases.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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