APCFire 750 anti-mouse H-2Kb bound to SIINFEKL Antibody, H-2Kb bound to SIINFEKL, 25-D116,BioLegend,141613

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3, and inhibition of T cell response to H-2Kb-SIINFEKLin vitro.

Host

Mouse

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB173528604

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameAPCFire 750 anti-mouse H-2Kb bound to SIINFEKL Antibody, H-2Kb bound to SIINFEKL, 25-D116
Cat. No.141613
HostMouse
RRIDAB_2750523 (BioLegend Cat. No. 141613)AB_2750524 (BioLegend Cat. No. 141614)
IsotypeMouse IgG1, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number25-D1.16
Concentration0.2 mg/ml
TargetH-2Kb bound to SIINFEKL
ImmunogenSIINFEKL pulsed RMA-S cells
PurityThe antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC)in vivo.

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