ATF-4 (D4B8) Rabbit Monoclonal Antibody#11815,Cell Signaling Technology (CST),11815

ATF-4 (D4B8) Rabbit Monoclonal Antibody recognizes endogenous levels of total ATF-4 protein. Reactivity by immunofluorescence is human only.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Chromatin IP: 1:200 Chromatin IP-seq: 1:200 CUT&RUN: 1:200 CUT&Tag: 1:200

Platform ID

BAB589351442

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameATF-4 (D4B8) Rabbit Monoclonal Antibody#11815
Cat. No.11815
Accession NumberP18848
Gene ID (Entrez)18848
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Chromatin IP: 1:200 Chromatin IP-seq: 1:200 CUT&RUN: 1:200 CUT&Tag: 1:200
Molecular Weight49
ImmunogenIgG
FormulationFor optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 106cells) per IP. This antibody has been validated using SimpleChIP®Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652.The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #82093.
Regulatory StatusResearch Use Only

Scientific Background

ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4).Fawcett, T.W. et al. (1999)Biochem J339 (Pt 1), 135-41.Harding, H.P. et al. (2000)Mol Cell6, 1099-108.van Huizen, R. et al. (2003)J Biol Chem278, 15558-64.Yusta, B. et al. (2006)Cell Metab4, 391-406.Alternate NamesActivating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB

Synonyms

Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB

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