ATTO 488 Anti-GFP antibody [1H1 / 1B2] - FluoTag®-X4,Abcam,AB325282

FluoTags® FluoTags are fully defined & ready-to-go fluorescent camelid single-domain antibody (sdAb) conjugates. All FluoTags® carry a defined number of fluorophores at known positions. Accordingly, they are available as FluoTag®-X2 or FluoTag®X4. Together with their monovalent binding mode, the defined fluorophore conjugation of FluoTags® results in a more quantitative and reliable readout in all fluorescence-based analyses. Due to the small size the fluorophore displacement from the bound target protein is very low and therefore FluoTags® are ideal for standard super resolution technologies. FluoTag®-X4 (One target - Two sdAb – Four fluorophores (1:2:4) Each FluoTag®-X4 reagent is a blend of two different sdAbs (FluoTag®-X2 and FluoTag®-X2-B) molecules that independently bind the target protein. As each sdAb carries two fluorophores per protein molecule, the blend can direct up to 4 fluorophores to each target protein. The reagent is therefore up to 2-fold brighter than the corresponding FluoTag®-X2 products. The maximal displacement of fluorophores from each antigen-binding surface is ~3 nm. FluoTag®-X4 reagents have the same size as the corresponding FluoTag®-X2 products and therefore show similarly good tissue penetration features. Features: A blend of two high affinity monovalent sdAbs directs four fluorophores to each target protein – ideal for bright signals Small size for optimal access to the respective target protein, also in crowded environment or 3D samples