Alexa Fluor® 488 Anti-HLTF antibody [EPR14761],Abcam,AB319500
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . How are conjugated primary antibodies validated? This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. For suitable applications and species reactivity, please refer to the unconjugated version of this clone. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Human
Application
Antibody Labelling, ICC/IF, IHC-P, Target Binding Affinity
Conjugate
Alexa Fluor® 488
Platform ID
BAB156269306

Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Functions as a DNA-dependent ATPase and E3 ubiquitin-protein ligase involved in chromatin regulation and DNA damage tolerance (DDT) (PubMed : 18316726, PubMed : 18719106, PubMed : 26051180, PubMed : 31960921, PubMed : 39142279, PubMed : 40680746). Catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164' in response to genotoxic stress, promoting error-free postreplication repair via template switching (PubMed : 18316726, PubMed : 18719106). Acts as an epigenetic regulator by promoting recruitment of DNMT1, thereby ensuring DNA methylation inheritance : specifically binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance (PubMed : 40680746). Catalyzes formation of H3K23ub in two steps : first mediates monoubiquitination together with UBE2E1 and UBE2D2, and then extends ubiquitin chains via 'Lys-63'-linked ubiquitination together with UBE2N and UBE2V2 (PubMed : 40680746). Also acts as a chromatin redodeling factor, thereby regulating transcription (PubMed : 10391891, PubMed : 1994885, PubMed : 9126292). Exhibits ATP-dependent double-stranded DNA (dsDNA) translocase activity but lacks classical helicase activity; mediates replication fork reversal by concertedly unwinding and annealing nascent and parental strands, thereby suppressing DNA synthesis and maintaining genomic stability (PubMed : 1994885). Resolves G-quadruplex (G4) DNA structures in cooperation with MSH2, limiting replication stress and G4 accumulation across the cell cycle (PubMed : 39142279). Contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides using its HIRAN and ATPase domains (PubMed : 26051180). Can displace single-stranded DNA from triplex structures through ATP-dependent dsDNA translocation (PubMed : 26051180, PubMed : 31960921). Also has protein clearing activity at the stalled replication fork, facilitating restart of DNA replication (PubMed : 21795603). See full target information HLTF
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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