Alexa Fluor® 488 Anti-mtTFA antibody [EPR12285] – Mitochondrial Marker,Abcam,AB317987

Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . How are conjugated primary antibodies validated? This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling. For suitable applications and species reactivity, please refer to the unconjugated version of this clone. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

Host

Rabbit

Reactivity

Human

Application

ICC/IF, Target Binding Affinity, IHC-P, Antibody Labelling

Conjugate

Alexa Fluor® 488

Platform ID

BAB970724617

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 488 Anti-mtTFA antibody [EPR12285] – Mitochondrial Marker
Cat. No.AB317987
HostRabbit
IsotypeIgG
ReactivityHuman
ConjugationAlexa Fluor® 488
ApplicationICC/IF, Target Binding Affinity, IHC-P, Antibody Labelling
ClonalityMonoclonal
Clone NumberEPR12285
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed : 29445193, PubMed : 32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed : 29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed : 20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed : 22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed : 22037172). Is able to unwind DNA (PubMed : 22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed : 1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed : 19304746, PubMed : 22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed : 22037171). See full target information TFAM

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