Alexa Fluor 488 anti-HA.11 Epitope Tag Antibody anti-HA.11 - 16B12,BioLegend,901509

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Host

Mouse

Application

FC - Quality testedICC - Verified

Platform ID

BAB043545968

BioLegend

Headquarters

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Contact

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 488 anti-HA.11 Epitope Tag Antibody anti-HA.11 - 16B12
Cat. No.901509
HostMouse
RRIDAB_2565072 (BioLegend Cat. No. 901509)
IsotypeMouse IgG1, κ
ApplicationFC - Quality testedICC - Verified
ClonalityMonoclonal
Clone Number16B12
Concentration1 mg/mL
TargetHA.11
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageStore between 2°C and 8°C.
Regulatory StatusResearch Use Only

Scientific Background

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

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