Alexa Fluor® 488 anti-Puromycin Antibody, Puromycin, 2A4,BioLegend,381505

It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase.For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.

Host

Mouse

Reactivity

Human, Mouse, Rat, All species

Application

ICFC - Quality tested

Platform ID

BAB613096361

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 488 anti-Puromycin Antibody, Puromycin, 2A4
Cat. No.381505
HostMouse
RRIDAB_2927825 (BioLegend Cat. No. 381505)AB_2927825 (BioLegend Cat. No. 381506)
IsotypeMouse IgG2a, κ
ReactivityHuman, Mouse, Rat, All species
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone Number2A4
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetPuromycin
ImmunogenPuromycin from Streptomyces alboniger coupled to KLH
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.

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