Alexa Fluor 488 anti-human SSEA-4 Antibody anti-SSEA-4 - MC-813-70,BioLegend,330411

Additional reported applications (for the relevant formats) include: ELISA1and immunohistochemistry1of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB126428694

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 488 anti-human SSEA-4 Antibody anti-SSEA-4 - MC-813-70
Cat. No.330411
HostMouse
RRIDAB_1089199 (BioLegend Cat. No. 330411)AB_1089198 (BioLegend Cat. No. 330412)
IsotypeMouse IgG3, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberMC-813-70
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
ImmunogenHuman embryonic carcinoma cell line 2102Ep
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

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