Alexa Fluor 488 anti-mouse CD86 Antibody anti-CD86 - GL-1,BioLegend,105018

The GL-1 antibody can block the mixed lymphocyte reactionin vitroand has been shown to inhibit the priming of cytotoxic T lymphocytesin vivo(along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, andin vivoandin vitroblocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB787834975

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 488 anti-mouse CD86 Antibody anti-CD86 - GL-1
Cat. No.105018
HostRat
RRIDAB_493463 (BioLegend Cat. No. 105017)AB_493462 (BioLegend Cat. No. 105018)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberGL-1
Concentration0.5 mg/ml
TargetCD86
ImmunogenLPS-activated CBA/Ca mouse splenic B cells
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.