Alexa Fluor 488 anti-mouse Ly-6G/Ly-6C Gr-1 Antibody anti-Ly-6G/Ly-6C - RB6-8C5,BioLegend,108419
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytesin vivo. Additional reported applications (for relevant formats of this clone) include:in vitrocomplement-mediated cytotoxicity2,in vivodepletion3-5,9, immunoprecipitation1, immunohistochemical staining6(including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.Special Note:Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).
Host
Rat
Reactivity
Mouse
Application
FC - Quality testedIHC - Reported in the literature, not verified in house
Platform ID
BAB771631492
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NameAlexa Fluor 488 anti-mouse Ly-6G/Ly-6C Gr-1 Antibody anti-Ly-6G/Ly-6C - RB6-8C5
Cat. No.108419
HostRat
RRIDAB_493480 (BioLegend Cat. No. 108419)AB_389309 (BioLegend Cat. No. 108417)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality testedIHC - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRB6-8C5
Concentration0.5 mg/ml
TargetLy-6G Ly-6C
ImmunogenRaised against granulocytes of mouse origin
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Category Paths
- Products>Primary Antibodies>IHC Antibodies
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