Alexa Fluor® 594 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker,Abcam,AB203429
Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Host
Rabbit
Reactivity
Human, Human, Mouse, Rat
Application
ICC/IF, Flow Cyt (Intra)
Conjugate
Alexa Fluor® 594
Platform ID
BAB679708050
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed : 15340068, PubMed : 15953362, PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22017874, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 33509017, PubMed : 34471133, PubMed : 34893540, PubMed : 35831301, PubMed : 37306101, PubMed : 37802024). Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps (PubMed : 16286508, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 34893540, PubMed : 37802024). SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies (PubMed : 15911346, PubMed : 20168092, PubMed : 22017874, PubMed : 24128730, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 37802024). SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins (PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 37802024). SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed : 16286508, PubMed : 17580304, PubMed : 37802024). Also required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed : 20168092). Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes (PubMed : 26344566). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1 : interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed : 20452972, PubMed : 28380357, PubMed : 33393215, PubMed : 37306101). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed : 29496741). Involved in endosome organization by retaining vesicles in the perinuclear cloud : following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed : 27368102, PubMed : 33472082). Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation (PubMed : 25101860). May regulate the activation of NFKB1 by TNF, nerve growth factor (NGF) and interleukin-1 (PubMed : 10356400, PubMed : 10747026, PubMed : 11244088, PubMed : 12471037, PubMed : 16079148, PubMed : 19931284). May play a role in titin/TTN downstream signaling in muscle cells (PubMed : 15802564). Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). See full target information SQSTM1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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