Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker,Abcam,AB154207

What is this antibody validated in? Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples. Trusted by the scientific community Anti-Vimentin [EPR3776] - Cytoskeleton Marker Alexa Fluor® 594 (ab154207) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals. Related products Antibody clone EPR3776 is also available pre-conjugated to a variety of labels for your convenience – Anti-Vimentin Alexa Fluor® 594 [EPR3776] - Cytoskeleton Marker (ab154207). Specificity confirmed The specificity of Alexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (ab154207) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR3776] also available for your convenience: ab92547 , Alexa Fluor® 594 - ab154207, Alexa Fluor® 488 - ab185030 , Carrier free - ab193555 , HRP - ab194718 , Alexa Fluor® 647 - ab194719 , Alexa Fluor® 568 - ab202504 , Alexa Fluor® 555 - ab203428 , PE - ab209446 , Alexa Fluor® 405 - ab210152 , Alexa Fluor® 568 - ab236866, Carrier free - ab307988, APC - ab314250 , ab316214 , Carrier free - ab316215 , Alexa Fluor® 750 - ab321763 , ab323238 , Carrier free - ab323247 What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

Host

Rabbit

Reactivity

Mouse, Human

Application

Flow Cyt (Intra), ICC/IF, IHC-P

Conjugate

Alexa Fluor® 594

Platform ID

BAB117976431

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 594 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker
Cat. No.AB154207
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ConjugationAlexa Fluor® 594
ApplicationFlow Cyt (Intra), ICC/IF, IHC-P
ClonalityMonoclonal
Clone NumberEPR3776
Concentration0.5 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. See full target information VIM

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