Alexa Fluor 594 anti-beta-actin Antibody anti-beta-actin - 2F1-1,BioLegend,643804

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommendedprotocol.

Host

Mouse

Reactivity

Human, Mouse, Rat

Application

ICC -Quality testedIHC-P -Verified

Platform ID

BAB990182859

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 594 anti-beta-actin Antibody anti-beta-actin - 2F1-1
Cat. No.643804
HostMouse
RRIDAB_2565573 (BioLegend Cat. No. 643804)
IsotypeMouse IgG2b, κ
ReactivityHuman, Mouse, Rat
ApplicationICC -Quality testedIHC-P -Verified
ClonalityMonoclonal
Clone Number2F1-1
Concentration0.5 mg/ml
Targetbeta-actin
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

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