Alexa Fluor® 647 anti-GATA1 Antibody, GATA1, P84F5,BioLegend,939105

This clone was tested for ICC using 4% PFA-fixed HEL cells permeabilized with either methanol or Triton X-100. While both methods were compatible with robust HEL staining, Triton X-100 permabilization resulted in stronger staining.When using this clone for ICFC, we recommend using the True-Nuclear™ Transcription Factor Buffer Set or the True Phos perm buffer. We do not recommend using the Intracellular Staining Permeabilization Wash Buffer for ICFC testing due to poor GATA1 staining.True-Nuclear™ Transcription Factor Buffer Set allowed for better binding to the epitope when compared to the True Phos perm buffer, there was a twofold difference between their median PE readings.

Host

Mouse

Reactivity

Human

Application

ICFC - Quality testedICC - Verified

Platform ID

BAB640671854

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 647 anti-GATA1 Antibody, GATA1, P84F5
Cat. No.939105
HostMouse
RRIDAB_3106235 (BioLegend Cat. No. 939105)AB_3106235 (BioLegend Cat. No. 939106)
IsotypeMouse IgG2b, κ
ReactivityHuman
ApplicationICFC - Quality testedICC - Verified
ClonalityMonoclonal
Clone NumberP84F5
Concentration0.5 mg/mL
TargetGATA1
ImmunogenFull-length recombinant human GATA1 protein
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The GATA family of transcription factors is comprised of six members characterized by their binding to the consensus DNA sequence (T/A)GATA(A/G). GATA1, which was the initially described member of the GATA family, is predominantly expressed in cells of the myeloid lineage such as erythrocytes, eosinophils, mast cells, and megacaryocytes. Over the course of erythroid differentiation, GATA2 activates expression of the functionally distinct GATA1; promoter-occupied GATA2 is replaced/exchanged at certain promoters with the GATA1 transcription factor, a process known as the GATA switch. Genes induced by GATA1 includeHBB,HBG1/2,ALAS2andHMBS. Haploinsufficient heterozygousGATA1knockout mice spontaneously develop erythroblastic leukemia, andGATA1-null mice die early in development due to profound anemia.

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