Alexa Fluor® 647 anti-GATA4 Antibody, GATA4, W19078B,BioLegend,948605

This clone was tested for ICC using HeLa cells and SH-SY5Y cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Both methods resulted in specific, but weak GATA4 staining. We therefore do not recommend this clone for the application.While this clone detected GATA4 by WB, it also showed a very weak affinity for the protein. Because of this, we do not recommend this clone for the application.This clone failed IHC testing due to lack of specificity for GATA4. We therefore do not recommend this clone for the application.This clone is not compatible with our True Nuclear™ Transcription Factor and Cyto-Fast™ buffer sets. We recommend using using True-Phos™ Perm Buffer (Cat. No. 425401) for ICFC.

Host

Rat

Reactivity

Human, Mouse

Application

ICFC - Quality tested

Platform ID

BAB145608219

BioLegend

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 647 anti-GATA4 Antibody, GATA4, W19078B
Cat. No.948605
HostRat
RRIDAB_2924630 (BioLegend Cat. No. 948605)AB_2924630 (BioLegend Cat. No. 948606)
IsotypeRat IgG2a, κ
ReactivityHuman, Mouse
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone NumberW19078B
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetGATA4
ImmunogenRecombinant human GATA4 protein
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

GATA binding protein 4 (GATA4) is a zinc-finger transcription factor that plays a crucial role in transcriptional activation and cell growth, differentiation, survival, and senescence. It is essential to cardiac development and function. Along with GATA6, it modulates cardiac stress response and regulates cardiomyocyte formation, replication, and function. GATA4 participates in second heart field transcription and differentiation and ventricular morphogenesis through downstream regulation of Mef2c and Hand2. Mutations in GATA4 can result in myocardial abnormalities, myocardial hypoplasia, absence of a proepicardium, abnormal valve formation, and endocardial cushion defects. In the intestine, GATA4, along with GATA6, is also responsible for regulating epithelial cytodifferentiation and structure, and for maintaining balance between enterocytes and secretory cells through the coordination of Notch signaling. GATA4 plays a vital role in human testicular development through its regulation of the expression of steroidogenic factors StAR, INHA, HSD3B2, and CYP19A1 and sex-determining genes SRY, AMH, and SOX9.

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