Alexa Fluor® 647 anti-HMOX1 Antibody, HMOX1, W19398C,BioLegend,934105

Clone was tested for ICC using three fix/perm methods (PFA/Triton-X, PFA/MeOH, MeOH only), the best method was the use of PFA/Triton X-100.In the CoCl2 treated cells for ICC, staining was seen in less than 5% of cells.Clone was tested for ICFC using cytokine fix/perm method only (based on ICC results).

Host

Rat

Reactivity

Human

Application

ICFC (test sizes) - Quality testedICC (µg size) - Quality tested

Platform ID

BAB459213938

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 647 anti-HMOX1 Antibody, HMOX1, W19398C
Cat. No.934105
HostRat
RRIDAB_2941637 (BioLegend Cat. No. 934105)AB_2941637 (BioLegend Cat. No. 934106)AB_2941637 (BioLegend Cat. No. 934108)
IsotypeRat IgG2a, κ
ReactivityHuman
ApplicationICFC (test sizes) - Quality testedICC (µg size) - Quality tested
ClonalityMonoclonal
Clone NumberW19398C
Concentrationµg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetHMOX1
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Formulationµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azidetest sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.