Alexa Fluor® 647 anti-TFEB Antibody, TFEB, W18197C,BioLegend,936105

The autophagy-lysosomal pathway is required for the quality control degradation of proteins and other macromolecules. The transcription factor EB (TFEB) plays a critical role in inducing expression of genes involved in autophagy and lysosomal biogenesis. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. Under nutrient-replete conditions, mTORC1 phosphorylates TFEB promoting its cytosolic retention by 14-3-3 proteins and nuclear export through CRM1. Under nutrient deprivation, TFEB phosphorylation decreases, promoting its nuclear import and transactivation function. TFEB is frequently dysregulated in cancer, and is also involved in the clearance of intracellular pathogenic targets in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.