Alexa Fluor 647 anti-Vimentin Antibody anti-Vimentin - O91D3,BioLegend,677807

While this clone recognizes mouse Vimentin, we do not recommend its usage for western blot due to poor affinity of the antibody for the protein. Additional reported applications for the relevant formats include: spatial biology (IBEX)1,2.

Host

Mouse

Reactivity

Human

Application

ICC - Quality testedIHC-P - VerifiedSB - Reported in the literature, not verified in house

Platform ID

BAB261486364

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 647 anti-Vimentin Antibody anti-Vimentin - O91D3
Cat. No.677807
HostMouse
RRIDAB_2616801 (BioLegend Cat. No. 677807)
IsotypeMouse IgG2a, κ
ReactivityHuman
ApplicationICC - Quality testedIHC-P - VerifiedSB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberO91D3
Concentration0.5 mg/mL
TargetVimentin
ImmunogenFull length human vimentin produced inE. coli.
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Vimentin are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is a widely expressed and highly conserved 54 kD protein that is constitutively expressed in mesenchymal cells, endothelial cells lining blood vessels, renal tubular cells, macrophages, neutrophils, fibroblasts, and leukocytes1,2. Vimentin is used as a marker of mesenchymal cells to distinguish them from epithelial cells3. Increased vimentin expression is frequently used as an EMT marker in cancer4. Autoantibodies to vimentin are commonly found in patients with autoimmune diseases such as Lupus5and rheumatoid arthritis6, and also found after transplantation7.

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