Alexa Fluor 647 anti-human CD16 Antibody anti-CD16 - B73.1,BioLegend,360713

The epitope recognized by clone B73.1 is in the first membrane distal Ig-like domain of the CD16 molecule, which is different from that of clone 3G84.Donor variability has been observed for clone B73.1 staining1, especially on granulocytes.

Host

Mouse

Reactivity

Human

Application

FC - Quality tested

Platform ID

BAB207912092

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

Email:

Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 647 anti-human CD16 Antibody anti-CD16 - B73.1
Cat. No.360713
HostMouse
RRIDAB_2563020 (BioLegend Cat. No. 360713)AB_2563021 (BioLegend Cat. No. 360714)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberB73.1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD16
ImmunogenNK cell-enriched fraction from human peripheral blood.
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, a subset of T cells and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.