Alexa Fluor 647 anti-human CD4 Antibody anti-CD4 - RPA-T4,BioLegend,300523
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
Host
Mouse
Reactivity
Human
Application
FC - Quality testedSB - Reported in the literature, not verified in house
Platform ID
BAB524804968
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameAlexa Fluor 647 anti-human CD4 Antibody anti-CD4 - RPA-T4
Cat. No.300523
HostMouse
RRIDAB_493097 (BioLegend Cat. No. 300523)AB_389333 (BioLegend Cat. No. 300520)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationFC - Quality testedSB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRPA-T4
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetCD4
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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