Alexa Fluor 647 anti-mouse CD31 Antibody anti-CD31 - MEC13.3,BioLegend,102516

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8Additional reported applications (in the relevant formats) include: immunoprecipitation1,in vitroandin vivoblocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.Special Note:The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No.102529 and 102530).

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested3D IHC -VerifiedSB -Reported in the literature, not verified in house

Platform ID

BAB814422207

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 647 anti-mouse CD31 Antibody anti-CD31 - MEC13.3
Cat. No.102516
HostRat
RRIDAB_2161030 (BioLegend Cat. No. 102515)AB_2161029 (BioLegend Cat. No. 102516)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested3D IHC -VerifiedSB -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMEC13.3
Concentration0.5 mg/mL
TargetCD31
ImmunogenPolyoma middle T transformed EC line tEnd.1
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αvβ3integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

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