Alexa Fluor 647 anti-mouse CD49e Antibody anti-CD49e - 5H10-27(MFR5),BioLegend,103811
Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7of acetone-fixed frozen sections, blocking of cell-cell adhesion1-5, inhibition of TNF-ß1costimulated T cell proliferation3, and costimulation of T cell proliferation by cross-linked 5H10-27 antibody3. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103817 and 103818).
Host
Rat
Reactivity
Mouse
Application
FC -Quality tested
Platform ID
BAB692869316
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameAlexa Fluor 647 anti-mouse CD49e Antibody anti-CD49e - 5H10-27(MFR5)
Cat. No.103811
HostRat
RRIDAB_528840 (BioLegend Cat. No. 103811)AB_528841 (BioLegend Cat. No. 103812)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number5H10-27(MFR5)
Concentration0.5 mg/ml
TargetCD49e
Immunogen(C57BL/6 x A/J)F1mouse mast cell line
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
CD49e is a 135 kD protein, also known as α5integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote thein vitrostimulation of CD8+T cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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