Alexa Fluor 647 anti-mouse H-2Kk Antibody anti-H-2Kk - 36-7-5,BioLegend,114912
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114914).Does not react with alloantigens of other haplotypes (e.g.,b, d, q).
Host
Mouse
Reactivity
Mouse
Application
FC -Quality tested
Platform ID
BAB181124956
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameAlexa Fluor 647 anti-mouse H-2Kk Antibody anti-H-2Kk - 36-7-5
Cat. No.114912
HostMouse
RRIDAB_493115 (BioLegend Cat. No. 114912)
IsotypeMouse (A.TL) IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number13336
Concentration0.5 mg/ml
TargetH-2Kk
ImmunogenA.AL mouse splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
The 36-7-5 antibody reacts with the H-2KkMHC class I alloantigen expressed on nucleated cells from mice of the H-2Kkhaplotype. H-2Kkis involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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