Alexa Fluor 647 anti-mouse I-Ab Antibody anti-I-Ab - KH74,BioLegend,115310
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2of acetone-fixed frozen tissue sections. Does not react with other haplotypes (e.g.,a, d, k, n, p, q, u).
Host
Mouse
Reactivity
Mouse
Application
FC -Quality tested
Platform ID
BAB617083182
BioLegend
Contact
Tel: 1-858-455-9588
Fax: +49 (4131) 7023913
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Product Specifications
Scientific Background
Specifications
NameAlexa Fluor 647 anti-mouse I-Ab Antibody anti-I-Ab - KH74
Cat. No.115310
HostMouse
RRIDAB_493139 (BioLegend Cat. No. 115309)AB_492826 (BioLegend Cat. No. 115310)
IsotypeMouse (BALB/c) IgG2a, κ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone NumberKH74
Concentration0.5 mg/ml
TargetI-Ab
ImmunogenC57BL/10 mouse splenocytes
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
The KH74 antibody reacts with the I-AbMHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2bbearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The KH74 antibody cross-reacts with the I-Af, I-Ar, I-As, and I-Avhaplotypes.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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