Alexa Fluor 647 anti-mouse/human CD15 SSEA-1 Antibody anti-SSEA-1 - MC-480,BioLegend,125608

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Host

Mouse

Reactivity

Human, Mouse

Application

FC - Quality tested

Platform ID

BAB563697555

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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor 647 anti-mouse/human CD15 SSEA-1 Antibody anti-SSEA-1 - MC-480
Cat. No.125608
HostMouse
RRIDAB_1089190 (BioLegend Cat. No. 125607)AB_1089188 (BioLegend Cat. No. 125608)
IsotypeMouse IgM, κ
ReactivityHuman, Mouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone NumberMC-480
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
ImmunogenMouse F9 Teratocarcinoma Stem Cells (X-irradiated)
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

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