Alexa Fluor® 700 anti-human IL-8 Antibody, IL-8, E8N1,BioLegend,511418

ELISA Detection:The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody.Note:For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.

Host

Mouse

Reactivity

Human

Application

ICFC -Quality tested

Platform ID

BAB117621659

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 700 anti-human IL-8 Antibody, IL-8, E8N1
Cat. No.511418
HostMouse
RRIDAB_2832819 (BioLegend Cat. No. 511417)AB_2832820 (BioLegend Cat. No. 511418)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationICFC -Quality tested
ClonalityMonoclonal
Clone NumberE8N1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIL-8
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.

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