Alexa Fluor® 700 anti-mouse/human Helios Antibody, Helios, 22F6,BioLegend,137242

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403).View more applications data for a different format of this clone in ourScientific Poster Library.

Host

Armenian Hamster

Reactivity

Mouse, Human

Application

ICFC -Quality tested

Platform ID

BAB047566566

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameAlexa Fluor® 700 anti-mouse/human Helios Antibody, Helios, 22F6
Cat. No.137242
HostArmenian Hamster
RRIDAB_2832496 (BioLegend Cat. No. 137241)AB_2832497 (BioLegend Cat. No. 137242)
IsotypeArmenian Hamster IgG
ReactivityMouse, Human
ApplicationICFC -Quality tested
ClonalityMonoclonal
Clone Number22F6
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetHelios
ImmunogenHelios peptide (aa 51-107)
PurityThe antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

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