Anti-6X His tag® antibody [HIS.H8],Abcam,AB18184

Anti-6X His tag ® antibody [HIS.H8] (ab18184) is a mouse monoclonal antibody and is validated for use in Dot, ELISA, ICC/IF, IP and WB. Anti-6X His tag ® antibody [HIS.H8] (ab18184) was first used in a scientific publication in 2004 and has been cited over 356 times in peer reviewed journals. It's performance in Western blot in human and mouse samples is trusted by the scientific community. Abcam's high quality validation processes ensure Anti-6X His tag ® antibody [HIS.H8] (ab18184) has high sensitivity and specificity. Anti-6X His tag ® antibody [HIS.H8] (ab18184) has 31 independent reviews from customers. 6X His tag ® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag ® antibody [HIS.H8] (ab18184) has been validated in Dot, ELISA, ICC/IF, IP and WB. Anti-6X His tag ® antibody [HIS.H8] (ab18184) specifically detects 6X His tag ® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 ug selling sizes. Antibody clone HIS.H8 is also available pre-conjugated to a variety of labels for your convenience - Biotin ( ab173828 ). Top cited 6X His tag antibody in the market with >450 citations. The 6X His tag, also known as a polyhistidine tag, is widely used for the purification of His-tagged proteins. Utilizing Ni-NTA chromatography, this tag facilitates efficient protein purification, ensuring high yield and purity. It is also highly relevant for detecting 6X His tags in multiple proteomic applications such as western blotting (WB), Immunoprecipitation (IP) and immunocytochemistry/immunofluorescence (ICC/IF) applications. HIS-TAG® is a trademark of EMD Biosciences, Inc.

Host

Mouse

Application

WB, ELISA, ICC/IF, Dot, IP

Platform ID

BAB642425954

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-6X His tag® antibody [HIS.H8]
Cat. No.AB18184
HostMouse
IsotypeIgG2b
ApplicationWB, ELISA, ICC/IF, Dot, IP
ClonalityMonoclonal
Clone NumberHIS.H8
Concentration1 mg/mL Batch dependent concentration
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data See full target information 6X His tag®

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