Anti-AIF antibody [E20] - Mitochondrial Marker,Abcam,AB32516
What is this antibody validated in? Anti-AIF antibody [E20] - Mitochondrial Marker (ab32516) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of AIF? Anti-AIF [E20] - Mitochondrial Marker (ab32516) specifically detects a band for AIF (UniProt: O95831) at a molecular weight of 67kDa. Trusted by the scientific community Anti-AIF [E20] - Mitochondrial Marker (ab32516) was first used in a scientific publication in 2006 and has been cited over 70 times in peer-reviewed journals. Reviewed by scientists Anti-AIF [E20] - Mitochondrial Marker (ab32516) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-AIF antibody [E20] - Mitochondrial Marker (ab32516) has been confirmed by Western blot testing in AIFM1 Knockout HEK-293 cells. Other related products We have a range of other formats of antibody clone [E20] also available for your convenience: ab32516, Alexa Fluor® 647 - ab196847 , Biotin - ab197526 , Carrier free - ab220215 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Human, Rat, Mouse
Application
IP, Flow Cyt (Intra), IHC-Fr, ICC/IF, IHC-P, WB
Platform ID
BAB273135712
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed : 17094969, PubMed : 20362274, PubMed : 23217327, PubMed : 33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed : 20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed : 20362274). Binds to DNA in a sequence-independent manner (PubMed : 27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed : 17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed : 19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed : 26004228).. Isoform 4. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.. Isoform 5. Pro-apoptotic isoform. See full target information AIFM1
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