Anti-ALKBH5 antibody [EPR18958],Abcam,AB195377
What is this antibody validated in? Anti-ALKBH5 antibody [EPR18958] (ab195377) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples. What is the molecular weight of ALKBH5? Anti-ALKBH5 [EPR18958] (ab195377) specifically detects a band for ALKBH5 (UniProt: Q6P6C2) at a molecular weight of 44kDa. Trusted by the scientific community Anti-ALKBH5 [EPR18958] (ab195377) was first used in a scientific publication in 2018 and has been cited over 60 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-ALKBH5 antibody [EPR18958] (ab195377) has been confirmed by Western blot testing in ALKBH5 Knockout HEK293T cell line, ab266762 . Other related products We have a range of other formats of antibody clone [EPR18958] also available for your convenience: ab195377, Carrier free - ab234528 , Alexa Fluor® 647 - ab245891 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
WB, IHC-P
Platform ID
BAB511654961
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed : 23177736, PubMed : 24489119, PubMed : 24616105, PubMed : 24778178, PubMed : 34048572, PubMed : 36944332, PubMed : 37257451, PubMed : 37369679). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (PubMed : 21264265, PubMed : 23177736, PubMed : 24489119, PubMed : 24616105, PubMed : 24778178). Demethylation of m6A mRNA affects mRNA processing, translation and export (PubMed : 23177736, PubMed : 34048572, PubMed : 36944332, PubMed : 37257451). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed : 24616105). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids : m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (PubMed : 37369679, PubMed : 37474102). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (PubMed : 37474102). Also acts as a negative regulator of T-cell development : inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (By similarity). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (By similarity). See full target information ALKBH5
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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