Anti-APC antibody [CC-1],Abcam,AB16794

Anti-APC antibody [CC-1] (ab16794) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-P in human samples. Anti-APC antibody [CC-1] (ab16794) has been cited over 130 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality validation processes ensure Anti-APC antibody [CC-1] (ab16794) has high sensitivity and specificity. Anti-APC antibody [CC-1] (ab16794) has 5 independent reviews from customers. Anti-APC antibody [CC-1] (ab16794) specifically detects APC (UniProt ID: P25054; Molecular weight: 312kDa) and is sold in 50 ug selling sizes. APC (Adenomatous Polyposis Coli) is a crucial tumor suppressor gene involved in regulating cell growth, adhesion, and division. APC protein interacts with beta-catenin, controlling its levels to prevent uncontrolled cell growth. APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody's staining of the cell body as opposed to the myelinated processes. Although we list several publications demonstrating mouse reactivity, we have also received conflicting data from researchers demonstrating a lack of mouse reactivity. Therefore, we have removed mouse from our list of reactive species and no longer guarantee the product for this species. This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Host

Mouse

Reactivity

Human

Application

ICC/IF, Flow Cyt, IHC-P

Platform ID

BAB329808496

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-APC antibody [CC-1]
Cat. No.AB16794
HostMouse
IsotypeIgG2b
ReactivityHuman
ApplicationICC/IF, Flow Cyt, IHC-P
ClonalityMonoclonal
Clone NumberCC-1
Concentration1 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human APC aa 1-250. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.05% Sodium azide Constituents: 1.902% Tris glycine, 0.8766% Sodium chloride
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Associates with both microtubules and actin filaments, components of the cytoskeleton (PubMed : 17293347). Plays a role in mediating the organization of F-actin into ordered bundles (PubMed : 17293347). Functions downstream of Rho GTPases and DIAPH1 to selectively stabilize microtubules (By similarity). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. See full target information APC

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