Anti-ATF3 antibody [EPR22610-19] - ChIP Grade,Abcam,AB254268

What is this antibody validated in? Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (ab254268) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse samples. What is the molecular weight of ATF3? Anti-ATF3 [EPR22610-19] - ChIP Grade (ab254268) specifically detects a band for ATF3 (UniProt: P18847) at a molecular weight of 21kDa. Trusted by the scientific community Anti-ATF3 [EPR22610-19] - ChIP Grade (ab254268) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (ab254268) has been confirmed by Immunocytochemistry testing in ATF3 Knockout HAP1 cell line, ab266955 . Other related products We have a range of other formats of antibody clone [EPR22610-19] also available for your convenience: ab254268, Carrier free - ab256534 , Alexa Fluor® 488 - ab319082 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Human

Application

ChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, IP, ChIC/CUT&RUN-seq

Platform ID

BAB405183563

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-ATF3 antibody [EPR22610-19] - ChIP Grade
Cat. No.AB254268
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ApplicationChIP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, IP, ChIC/CUT&RUN-seq
ClonalityMonoclonal
Clone NumberEPR22610-19
Concentration0.573 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data This protein binds the cAMP response element (CRE) (consensus : 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter.. Isoform 2. Activates transcription presumably by sequestering inhibitory cofactors away from the promoters.. Isoform 3. Stress-induced isoform, counteracts the transcriptional repression of isoform 1. See full target information ATF3

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