Anti-Acid sphingomyelinase antibody [EPR23090-4] - BSA and Azide free (Detector),Abcam,AB277244
ab277244 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Acid sphingomyelinase. The recommended pair for sandwich ELISA is: Capture: ab277243 , Human Acid sphingomyelinase Capture Antibody (unconjugated) Detector: ab277244, Human Acid sphingomyelinase Detector Antibody (unconjugated) The reference range value is 125 - 8000 pg/ml. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Human
Application
sELISA
Platform ID
BAB644524946
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Converts sphingomyelin to ceramide (PubMed : 12563314, PubMed : 1840600, PubMed : 18815062, PubMed : 25339683, PubMed : 25920558, PubMed : 27659707, PubMed : 33163980). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed : 20807762, PubMed : 21098024, PubMed : 9660788). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed : 12563314, PubMed : 20530211, PubMed : 20807762, PubMed : 22573858, PubMed : 9393854).. In the lysosomes, converts sphingomyelin to ceramide (PubMed : 20807762, PubMed : 21098024). Plays an important role in the export of cholesterol from the intraendolysosomal membranes (PubMed : 25339683). Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol (PubMed : 25339683). Modulates stress-induced apoptosis through the production of ceramide (PubMed : 8706124).. When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide (PubMed : 12563314, PubMed : 17303575, PubMed : 20807762). Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses (PubMed : 12563314, PubMed : 20807762, PubMed : 9393854). Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense (PubMed : 12563314, PubMed : 20807762, PubMed : 9393854). Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P. aeruginosa, induce apoptosis and regulate the cytokine response in infected cells (PubMed : 12563314). In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair (PubMed : 20530211).. Sphingomyelin phosphodiesterase, processed form. This form is generated following cleavage by CASP7 in the extracellular milieu in response to bacterial infection (PubMed : 21157428). It shows increased ability to convert sphingomyelin to ceramide and promotes plasma membrane repair (By similarity). Plasma membrane repair by ceramide counteracts the action of gasdermin-D (GSDMD) perforin (PRF1) pores that are formed in response to bacterial infection (By similarity).. (Microbial infection) Secretion is activated by bacteria such as P. aeruginos, N. gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.. (Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.. Isoform 2. Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.. Isoform 3. Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity. See full target information SMPD1
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