Anti-Argonaute-2 antibody [EPR10411],Abcam,AB186733
What is this antibody validated in? Anti-Argonaute-2 antibody [EPR10411] (ab186733) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Argonaute-2? Anti-Argonaute-2 [EPR10411] (ab186733) specifically detects a band for Argonaute-2 (UniProt: Q9UKV8) at a molecular weight of 97kDa. Trusted by the scientific community Anti-Argonaute-2 [EPR10411] (ab186733) was first used in a scientific publication in 2014 and has been cited over 230 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Argonaute-2 antibody [EPR10411] (ab186733) has been confirmed by Western blot testing in AGO2 Knockout HCT 116 cells. Other related products We have a range of other formats of antibody clone [EPR10411] also available for your convenience: ab186733, Alexa Fluor® 488 - ab206104 , Carrier free - ab233727 , PE - ab305668 , APC - ab305669 , HRP - ab305670 , Alkaline Phosphatase - ab308777 , Alexa Fluor® 647 - ab310088 , Alexa Fluor® 594 - ab310492 , Alexa Fluor® 555 - ab312021 , Alexa Fluor® 568 - ab312498 , Alexa Fluor® 750 - ab321299 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IHC-P, ICC/IF, WB, Flow Cyt (Intra), IP
Platform ID
BAB465066070
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.. (Microbial infection) Upon Sars-CoV-2 infection, associates with viral miRNA-like small RNA, CoV2-miR-O7a, and may repress mRNAs, such as BATF2, to evade the IFN response. See full target information AGO2
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