Anti-BRD2 antibody [EPR7642] - ChIP Grade,Abcam,AB139690
What is this antibody validated in? Anti-BRD2 antibody [EPR7642] - ChIP Grade (ab139690) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human samples. What is the molecular weight of BRD2? Anti-BRD2 [EPR7642] - ChIP Grade (ab139690) specifically detects a band for BRD2 (UniProt: P25440) at a molecular weight of 88kDa. Trusted by the scientific community Anti-BRD2 [EPR7642] - ChIP Grade (ab139690) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-BRD2 antibody [EPR7642] - ChIP Grade (ab139690) has been confirmed by Western blot testing in BRD2 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR7642] also available for your convenience: ab139690, Alexa Fluor® 488 - ab197865 , Alexa Fluor® 647 - ab197866 , HRP - ab198536 , Alexa Fluor® 555 - ab213347 , Alexa Fluor® 594 - ab215507 , Carrier free - ab222393 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Human
Application
Flow Cyt (Intra), ChIP, WB, ICC/IF, IHC-P, IP
Platform ID
BAB169351759
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 17148447, PubMed : 17848202, PubMed : 18406326, PubMed : 20048151, PubMed : 20709061, PubMed : 20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin : recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed : 35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed : 28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 : recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed : 28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed : 37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed : 37731000). See full target information BRD2
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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