Anti-CASC5 antibody [HL2158],Abcam,AB317147

Target data Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed : 15502821, PubMed : 17981135, PubMed : 18045986, PubMed : 19893618, PubMed : 21199919, PubMed : 22000412, PubMed : 22331848, PubMed : 27881301, PubMed : 30100357). Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules (PubMed : 18045986, PubMed : 19893618, PubMed : 27881301). The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 38459127, PubMed : 38459128). Required for kinetochore binding by a distinct subset of kMAPs (kinetochore-bound microtubule-associated proteins) and motors (PubMed : 19893618). Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (PubMed : 18045986, PubMed : 27881301). Can bind either to microtubules or to the protein phosphatase 1 (PP1) catalytic subunits PPP1CA and PPP1CC (via overlapping binding sites), it has higher affinity for PP1 (PubMed : 30100357). Recruits MAD2L1 to the kinetochore and also directly links BUB1 and BUB1B to the kinetochore (PubMed : 17981135, PubMed : 19893618, PubMed : 22000412, PubMed : 22331848, PubMed : 25308863). In addition to orienting mitotic chromosomes, it is also essential for alignment of homologous chromosomes during meiotic metaphase I (By similarity). In meiosis I, required to activate the spindle assembly checkpoint at unattached kinetochores to correct erroneous kinetochore-microtubule attachments (By similarity). See full target information KNL1